基于SELDI-TOF-MS技术的患亚临床低血钙症奶牛血浆蛋白质组谱分析

doi:10.11843/j.issn.0366-6964.2014.11.022

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1895-1903.doi: 10.11843/j.issn.0366-6964.2014.11.022

基于SELDI-TOF-MS技术的患亚临床低血钙症奶牛血浆蛋白质组谱分析

王朋贤¹，舒适¹，王博¹，许楚楚¹，刘健男¹，夏成^1*，吴凌¹，王哲²

（1.黑龙江八一农垦大学动物科技学院，大庆 163319；2.吉林大学畜牧兽医学院，长春130062）

收稿日期:2014-04-04 出版日期:2014-11-23 发布日期:2014-11-23
通讯作者: 夏成（1964-），男，教授，博导，主要从事奶牛营养代谢病研究，E-mail：xcwlxyf@sohu.com，Tel：0459-6819207
作者简介:王朋贤（1988-），女，河北石家庄人，硕士生，主要从事奶牛营养代谢病研究，E-mail：wpx519@163.com
基金资助:
国家自然基金面上项目（30972235）；“十二五”国家科技部项目（2013BAD21B01-2；2012BAD12B05-2；2012BAD12B03-2）

SELDI-TOF-MS Analysis of the Post-parturition Plasma Proteomic Profile of Dairy Cows with Subclinical Hypocalcemia

WANG Peng-xian¹，SHU Shi¹，WANG Bo¹，XU Chu-chu¹，LIU Jian-nan¹，XIA Cheng^1*，WU Ling¹，WANG Zhe²

(1.College of Animal Science and Veterinary Medicine，Heilongjiang Bayi Agricultural University，Daqing 163319，China；2.College of Animal Science and Veterinary Medicine，Jilin University，Changchun 130062，China)

Received:2014-04-04 Online:2014-11-23 Published:2014-11-23

摘要/Abstract

摘要：

旨在探讨患亚临床低血钙症奶牛血浆蛋白质组学的异常变化。收集32头患亚临床低血钙症奶牛和59头健康奶牛的血浆，应用SELDI-TOF-MS技术测得血浆蛋白质谱，经wilcoxon sum rank test分析两组峰值，结合Swissport 蛋白质数据库鉴定，从而获得组间差异表达蛋白，并进行决策树分析。结果获得了7个差异峰，经鉴定得到6种差异表达蛋白质。经决策树模型分析得出神经分泌蛋白VGF片段和淀粉样β蛋白4可能为诊断健康牛和亚临床低血钙症牛的生物标志物。应用SELDI-TOF-MS技术可有效分离健康牛与患病牛之间的血浆差异表达蛋白质，其中有可能存在能够成为该疾病的诊断标识物，其具体发生机制有待研究，该结果对探究奶牛亚临床低血钙症发病机制及其对机体生物学功能的影响具有重要的理论价值。

Abstract:

The aim of this study was to determine the differences between the post-parturition plasma proteomic profiles of healthy cows and those with subclinical hypocalcemia (SH).Plasma samples were obtained from 32 dairy cows with SH (group T) and 59 dairy cows without SH (group C).Plasma proteins were tested by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).The peaks that differ in the two groups were analyzed using the Wilcoxon sum-rank test.The differential peptide peaks were identified using the Swissprot protein database，which were performed by decision tree analysis.Seven peptide peaks were identified in the group T that differed significantly from those of the group C，which represented 6 unique proteins.Further，neurosecretory protein VGF frag and amyloid β A4 protein could be used for discriminate the cows with SH from the health after decision tree analysis.The SELDI-TOF-MS technology can effectively identify differences in serum protein expression patterns in cows with SH，which may become biomarkers for disease diagnosis.Therefore，this study has important theoretical value to reveal new mechanism of subclinical hypocalcemia and its effect on bio-function of dairy cows.

中图分类号:

S856.5

王朋贤，舒适，王博，许楚楚，刘健男，夏成，吴凌，王哲. 基于SELDI-TOF-MS技术的患亚临床低血钙症奶牛血浆蛋白质组谱分析[J]. 畜牧兽医学报, 2014, 45(11): 1895-1903.

WANG Peng-xian，SHU Shi，WANG Bo，XU Chu-chu，LIU Jian-nan，XIA Cheng，WU Ling，WANG Zhe. SELDI-TOF-MS Analysis of the Post-parturition Plasma Proteomic Profile of Dairy Cows with Subclinical Hypocalcemia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1895-1903.

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基于SELDI-TOF-MS技术的患亚临床低血钙症奶牛血浆蛋白质组谱分析

SELDI-TOF-MS Analysis of the Post-parturition Plasma Proteomic Profile of Dairy Cows with Subclinical Hypocalcemia

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